Abstract
Pyridoxal 4-dehydrogenase catalyzes the irreversible oxidation of pyridoxal to 4-pyridoxolactone and is involved in degradation pathway I of pyridoxine, a vitamin B(6) compound. Its crystal structure was elucidated for the first time. Molecular replacement with (S)-1-phenylthanol dehydrogenase (PDB code 2EW8) was adopted to determine the tertiary structure of the NAD(+)-bound enzyme.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alcohol Oxidoreductases / chemistry*
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Alcohol Oxidoreductases / metabolism
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Crystallography, X-Ray
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Models, Molecular
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Oxidoreductases / chemistry
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Oxidoreductases / metabolism
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Protein Conformation
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Proteobacteria / enzymology*
Substances
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Oxidoreductases
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Alcohol Oxidoreductases
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pyridoxal dehydrogenase
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1-phenylethanol dehydrogenase