The crystal structure of SDR-type pyridoxal 4-dehydrogenase of Mesorhizobium loti

Biosci Biotechnol Biochem. 2011;75(2):388-90. doi: 10.1271/bbb.100748. Epub 2011 Feb 7.

Abstract

Pyridoxal 4-dehydrogenase catalyzes the irreversible oxidation of pyridoxal to 4-pyridoxolactone and is involved in degradation pathway I of pyridoxine, a vitamin B(6) compound. Its crystal structure was elucidated for the first time. Molecular replacement with (S)-1-phenylthanol dehydrogenase (PDB code 2EW8) was adopted to determine the tertiary structure of the NAD(+)-bound enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / chemistry*
  • Alcohol Oxidoreductases / metabolism
  • Crystallography, X-Ray
  • Models, Molecular
  • Oxidoreductases / chemistry
  • Oxidoreductases / metabolism
  • Protein Conformation
  • Proteobacteria / enzymology*

Substances

  • Oxidoreductases
  • Alcohol Oxidoreductases
  • pyridoxal dehydrogenase
  • 1-phenylethanol dehydrogenase