A calcium-dependent xylan-binding domain of alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1

Biosci Biotechnol Biochem. 2011;75(2):379-81. doi: 10.1271/bbb.100730. Epub 2011 Feb 7.

Abstract

Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca(2+)-dependent xylan-binding, and that Asp313 and Asp318 were especially important.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / enzymology*
  • Calcium / metabolism*
  • Endo-1,4-beta Xylanases / chemistry*
  • Endo-1,4-beta Xylanases / genetics
  • Endo-1,4-beta Xylanases / metabolism*
  • Models, Molecular
  • Mutation
  • Protein Structure, Tertiary
  • Xylans / metabolism*

Substances

  • Xylans
  • Endo-1,4-beta Xylanases
  • Calcium