Abstract
Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca(2+)-dependent xylan-binding, and that Asp313 and Asp318 were especially important.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus / enzymology*
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Calcium / metabolism*
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Endo-1,4-beta Xylanases / chemistry*
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Endo-1,4-beta Xylanases / genetics
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Endo-1,4-beta Xylanases / metabolism*
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Models, Molecular
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Mutation
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Protein Structure, Tertiary
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Xylans / metabolism*
Substances
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Xylans
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Endo-1,4-beta Xylanases
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Calcium