Chronic exposure to arsenite induces S100A8 and S100A9 expression in rat RBL-2H3 mast cells

J Toxicol Sci. 2011 Jan;36(1):135-9. doi: 10.2131/jts.36.135.

Abstract

To investigate the effects of chronic exposure to arsenite on the gene expression profiles of mast cells, microarray analysis was performed on rat basophilic leukemia RBL-2H3 cells exposed to arsenite for 28 days. Upregulated genes include calcium-binding S100 proteins such as S100A9, S100A10, S100A6, and S100A13, and granzymes B and C. Among S100 proteins, S100A9 showed the highest expression (8.62-fold of untreated cells) after 4-weeks of exposure to arsenite. As S100A8 and S100A9 comprise a heterodimer called calprotectin, and are implicated in the development of atherosclerosis and cancer, mRNA levels of both S100A8 and S100A9 were analyzed. The results demonstrated that exposure of RBL-2H3 cells to arsenite for a few weeks induces marked increases in mRNA levels of S100A8 and S100A9.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arsenites / toxicity*
  • Atherosclerosis / etiology
  • Calgranulin A / genetics*
  • Calgranulin B / genetics*
  • Gene Expression Profiling*
  • Leukemia, Basophilic, Acute
  • Mast Cells / drug effects*
  • Mast Cells / metabolism*
  • Microarray Analysis
  • Neoplasms / etiology
  • RNA, Messenger / metabolism*
  • Rats
  • Sodium Compounds / toxicity*
  • Time Factors
  • Tumor Cells, Cultured
  • Up-Regulation
  • Water Pollutants, Chemical / toxicity*

Substances

  • Arsenites
  • Calgranulin A
  • Calgranulin B
  • RNA, Messenger
  • Sodium Compounds
  • Water Pollutants, Chemical
  • sodium arsenite