N-α-acetyltransferase 10 protein suppresses cancer cell metastasis by binding PIX proteins and inhibiting Cdc42/Rac1 activity

Cancer Cell. 2011 Feb 15;19(2):218-31. doi: 10.1016/j.ccr.2010.11.010. Epub 2011 Feb 3.

Abstract

N-α-acetyltransferase 10 protein, Naa10p, is an N-acetyltransferase known to be involved in cell cycle control. We found that Naa10p was expressed lower in varieties of malignancies with lymph node metastasis compared with non-lymph node metastasis. Higher Naa10p expression correlates the survival of lung cancer patients. Naa10p significantly suppressed migration, tumor growth, and metastasis independent of its enzymatic activity. Instead, Naa10p binds to the GIT-binding domain of PIX, thereby preventing the formation of the GIT-PIX-Paxillin complex, resulting in reduced intrinsic Cdc42/Rac1 activity and decreased cell migration. Forced expression of PIX in Naa10-transfected tumor cells restored the migration and metastasis ability. We suggest that Naa10p functions as a tumor metastasis suppressor by disrupting the migratory complex, PIX-GIT- Paxillin, in cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / metabolism
  • Acetyltransferases / physiology*
  • Aged
  • Cell Movement
  • Female
  • Guanine Nucleotide Exchange Factors / metabolism*
  • Humans
  • Lung Neoplasms / enzymology
  • Lung Neoplasms / metabolism
  • Lung Neoplasms / pathology*
  • Male
  • Middle Aged
  • N-Terminal Acetyltransferase A
  • N-Terminal Acetyltransferase E
  • Neoplasm Metastasis / prevention & control*
  • Rho Guanine Nucleotide Exchange Factors
  • cdc42 GTP-Binding Protein / antagonists & inhibitors*
  • rac1 GTP-Binding Protein / antagonists & inhibitors*

Substances

  • Guanine Nucleotide Exchange Factors
  • Rho Guanine Nucleotide Exchange Factors
  • Acetyltransferases
  • N-Terminal Acetyltransferase A
  • NAA10 protein, human
  • N-Terminal Acetyltransferase E
  • cdc42 GTP-Binding Protein
  • rac1 GTP-Binding Protein