Abstract
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA Primers* / genetics
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Humans
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Picornaviridae Infections / diagnosis*
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Picornaviridae Infections / epidemiology
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Picornaviridae Infections / virology
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Polymerase Chain Reaction / methods*
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RNA, Viral / genetics
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Rhinovirus / classification
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Rhinovirus / genetics
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Rhinovirus / isolation & purification*
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Sensitivity and Specificity
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Species Specificity