Imipenem disc for detection of KPC carbapenemase-producing Enterobacteriaceae in clinical practice

Shmuel Benenson; Violeta Temper; Matan J Cohen; Carmela Schwartz; Carlos Hidalgo-Grass; Colin Block

doi:10.1128/JCM.02179-10

Imipenem disc for detection of KPC carbapenemase-producing Enterobacteriaceae in clinical practice

J Clin Microbiol. 2011 Apr;49(4):1617-20. doi: 10.1128/JCM.02179-10. Epub 2011 Feb 2.

Authors

Shmuel Benenson¹, Violeta Temper, Matan J Cohen, Carmela Schwartz, Carlos Hidalgo-Grass, Colin Block

Affiliation

¹ Department of Clinical Microbiology and Infectious Diseases, Hadassah-Hebrew University Medical Center, P.O. Box 12000, Jerusalem 91120, Israel. Benenson@Hadassah.org.il

Abstract

The global spread of class A-carbapenemase-producing Enterobacteriaceae has made the development of a simple test a desirable goal. A disc diffusion test using imipenem was 100% sensitive and 96% specific in identifying carbapenemase-producing organisms, potentially reducing or eliminating the need for the relatively labor-intensive modified Hodge test.

Publication types

Evaluation Study

MeSH terms

Anti-Bacterial Agents*
Bacterial Proteins / analysis*
Bacteriological Techniques / methods*
Enterobacteriaceae / enzymology*
Enterobacteriaceae / isolation & purification
Enterobacteriaceae Infections / microbiology*
Humans
Imipenem*
Sensitivity and Specificity
beta-Lactamases / analysis*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Imipenem
beta-Lactamases
carbapenemase