Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution

Jing Ge; Zhaofang Liu; Zeyong Zhi; Xin Sheng Zhao

doi:10.1016/j.ab.2011.01.032

Qualitative and quantitative thrombin analysis in serum using aptamers and gold nanoparticles through progressive dilution

Anal Biochem. 2011 Jun 1;413(1):75-7. doi: 10.1016/j.ab.2011.01.032. Epub 2011 Feb 1.

Authors

Jing Ge¹, Zhaofang Liu, Zeyong Zhi, Xin Sheng Zhao

Affiliation

¹ Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

PMID: 21284928
DOI: 10.1016/j.ab.2011.01.032

Abstract

It has been a long-standing challenge in bioassay using aptamers and gold nanoparticles to detect disease-related proteins and other substance directly in complex biological samples such as serum. Here we propose a progressive dilution (PD) method to achieve simultaneous qualitative and quantitative analysis of proteins in blood serum without pretreatment of the sample. Above the detection limit, PD has unlimited dynamic range. We demonstrate the PD strategy through the detection of thrombin in fetal bovine serum using the quenching of fluorescence by gold nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aptamers, Nucleotide / chemistry*
Cattle
Gold / chemistry*
Metal Nanoparticles / chemistry*
Spectrometry, Fluorescence / methods*
Thrombin / analysis*

Substances

Aptamers, Nucleotide
Gold
Thrombin