Abstract
A proteomic approach has enabled the identification of an orthologue of the splicing factor hnRNP G in the amphibians Xenopus tropicalis, Ambystoma mexicanum, Notophthalmus viridescens and Pleurodeles walt, which shows a specific RNA-binding affinity similar to that of the human hnRN G protein. Three isoforms of this protein with a differential binding affinity for a specific RNA probe were identified in the P. walt oocyte. In situ hybridization to lampbrush chromosomes of P. waltl revealed the presence of a family of hnRNP G genes, which were mapped on the Z and W chromosomes and one autosome. This indicates that the isoforms identified in this study are possibly encoded by a gene family linked to the evolution of sex chromosomes similarly to the hnRNP G/RBMX gene family in mammals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amphibian Proteins / chemistry
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Amphibian Proteins / genetics
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Amphibian Proteins / metabolism*
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Animals
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Female
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Genotype
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Heterogeneous-Nuclear Ribonucleoprotein Group F-H / chemistry
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Heterogeneous-Nuclear Ribonucleoprotein Group F-H / genetics
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Heterogeneous-Nuclear Ribonucleoprotein Group F-H / metabolism*
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Heterogeneous-Nuclear Ribonucleoproteins / chemistry
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Humans
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Multigene Family
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Oocytes / metabolism
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Peptides / chemistry
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Peptides / metabolism
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Pleurodeles / genetics
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA Probes
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Sex Chromosomes
Substances
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Amphibian Proteins
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Heterogeneous-Nuclear Ribonucleoprotein Group F-H
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Heterogeneous-Nuclear Ribonucleoproteins
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Peptides
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Protein Isoforms
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RBMX protein, human
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RNA Probes