Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast

J Clin Microbiol. 2011 Apr;49(4):1614-6. doi: 10.1128/JCM.02381-10. Epub 2011 Jan 26.

Abstract

Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry was compared to phenotypic testing for yeast identification. MALDI-TOF mass spectrometry yielded 96.3% and 84.5% accurate species level identifications (spectral scores, ≥ 1.8) for 138 common and 103 archived strains of yeast. MALDI-TOF mass spectrometry is accurate, rapid (5.1 min of hands-on time/identification), and cost-effective ($0.50/sample) for yeast identification in the clinical laboratory.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Clinical Laboratory Techniques / economics
  • Clinical Laboratory Techniques / methods*
  • Costs and Cost Analysis
  • Humans
  • Mycology / economics
  • Mycology / methods*
  • Mycoses / diagnosis*
  • Mycoses / microbiology
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / economics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Time Factors
  • Yeasts / chemistry
  • Yeasts / classification
  • Yeasts / isolation & purification*