Oligonucleotide labeling using BODIPY phosphoramidite

Nucleosides Nucleotides Nucleic Acids. 2011 Jan;30(1):1-11. doi: 10.1080/15257770.2010.536798.

Abstract

4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivatives were prepared and their photochemical properties were characterized. One such analogue, 4,4-difluoro-4-bora-1,3,5,7-tetramethyl-8-(5-hydroxypentyl)-3a,4a-diaza-s-indacene was transformed into the corresponding phosphoramidite and incorporated into oligodeoxyribonucleotides as a fluorescent reporter group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Boron Compounds / analysis
  • Boron Compounds / chemical synthesis*
  • Fluorescent Dyes / chemistry*
  • Oligodeoxyribonucleotides / analysis
  • Oligodeoxyribonucleotides / chemistry*
  • Organophosphorus Compounds / analysis
  • Organophosphorus Compounds / chemistry*

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Boron Compounds
  • Fluorescent Dyes
  • Oligodeoxyribonucleotides
  • Organophosphorus Compounds
  • phosphoramidite