The presence of TRAIL-OPG complex in human osteosarcoma and human salivary gland adenocarcinoma

J Immunoassay Immunochem. 2011;32(1):70-8. doi: 10.1080/15321819.2010.538110.

Abstract

A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for the human tumor necrosis factor-related apoptosis-induced ligand (TRAIL)-osteoprotegerin (OPG) complex was developed by utilizing a monoclonal antibody that recognizes human soluble TRAIL as an immobilized capture component and anti-human OPG immunoglobulin G (IgG) labeled with biotin. We could quantify the TRAIL-OPG complex level (detection limit: 10 pmol/L). Employing this assay system, we demonstrated that the TRAIL-OPG complex was constitutively present in the human osteosarcoma cell lines, HOS, MG-63, and human salivary gland adenocarcinoma cell line, HSG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / immunology*
  • Antibodies, Monoclonal / immunology
  • Cell Line, Tumor
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Osteosarcoma / immunology*
  • Osteosarcoma / metabolism
  • RANK Ligand / analysis
  • RANK Ligand / immunology*
  • Salivary Gland Neoplasms / immunology*
  • TNF-Related Apoptosis-Inducing Ligand / analysis
  • TNF-Related Apoptosis-Inducing Ligand / immunology*

Substances

  • Antibodies, Monoclonal
  • RANK Ligand
  • TNF-Related Apoptosis-Inducing Ligand