Two-photon fluorescence lysosomal bioimaging with a micelle-encapsulated fluorescent probe

J Fluoresc. 2011 May;21(3):1223-30. doi: 10.1007/s10895-010-0801-3. Epub 2011 Jan 18.

Abstract

We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic® F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Survival
  • Fluorescence*
  • Fluorescent Dyes / chemical synthesis*
  • Humans
  • Lysosomes / metabolism*
  • Micelles*
  • Molecular Imaging / methods*
  • Poloxamer

Substances

  • Fluorescent Dyes
  • Micelles
  • Poloxamer