Ultrasensitive scanometric strategy for detection of matrix metalloproteinases using a histidine tagged peptide-Au nanoparticle probe

Wei Cheng; Yunlong Chen; Feng Yan; Lin Ding; Shijia Ding; Huangxian Ju; Yibing Yin

doi:10.1039/c0cc04441e

Ultrasensitive scanometric strategy for detection of matrix metalloproteinases using a histidine tagged peptide-Au nanoparticle probe

Chem Commun (Camb). 2011 Mar 14;47(10):2877-9. doi: 10.1039/c0cc04441e. Epub 2011 Jan 18.

Authors

Wei Cheng¹, Yunlong Chen, Feng Yan, Lin Ding, Shijia Ding, Huangxian Ju, Yibing Yin

Affiliation

¹ Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P.R. China.

PMID: 21243170
DOI: 10.1039/c0cc04441e

Abstract

A simple scanometric strategy was developed for ultrasensitive assay of matrix metalloproteinases based on their discriminatory and proteolytic activity by integrating a newly designed peptide-gold nanoparticle probe, a nitrilotriacetic acid modified chip and silver signal amplification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Biosensing Techniques / methods*
Gold / chemistry*
Histidine / metabolism*
Matrix Metalloproteinase 7 / metabolism
Matrix Metalloproteinases / metabolism*
Metal Nanoparticles / chemistry*
Molecular Sequence Data
Nitrilotriacetic Acid / chemistry
Peptides / chemistry*
Peptides / metabolism*
Substrate Specificity

Substances

Peptides
Histidine
Gold
Matrix Metalloproteinases
Matrix Metalloproteinase 7
Nitrilotriacetic Acid