A prototype of giant magnetoimpedance-based biosensing system for targeted detection of gastric cancer cells

Biosens Bioelectron. 2011 Mar 15;26(7):3246-53. doi: 10.1016/j.bios.2010.12.034. Epub 2010 Dec 29.

Abstract

A targeted detection of gastric cancer cells is achieved by combining the giant magnetoimpedance (GMI)-based biosensing system and RGD-4C peptide coupled, chitosan covered superparamagnetic iron oxide particles (RGD-Fe(3)O(4)@chitosan). The micro-patterned GMI sensor for targeted detection is made of Co-based ribbon and fabricated by micro electromechanical system (MEMS) technology. Functionalized nanoparticles were designed by coating Fe(3)O(4) with chitosan and conjugating with RGD-4C peptides. The targeted cells were trickled down into the detection area of the system. The detection of each sample is carried out in ten-fold manner and average value is taken as the final result. This system can identify the differences between targeted cells and non-targeted cells. It is of considerable interest due to its potential application in the biomedical field of various specific detections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Cell Line
  • Chitosan* / chemistry
  • Equipment Design
  • Ferric Compounds* / chemistry
  • Humans
  • Magnetics / instrumentation*
  • Nanoparticles* / chemistry
  • Nanoparticles* / ultrastructure
  • Oligopeptides* / chemistry
  • Stomach Neoplasms / diagnosis*

Substances

  • Ferric Compounds
  • Oligopeptides
  • ferric oxide
  • arginyl-glycyl-aspartic acid
  • Chitosan