Construction of recombinant mercury resistant Acidithiobacillus caldus

Dandan Chen; Jianqun Lin; Yuanyuan Che; Xiangmei Liu; Jianqiang Lin

doi:10.1016/j.micres.2010.10.003

Construction of recombinant mercury resistant Acidithiobacillus caldus

Microbiol Res. 2011 Oct 20;166(7):515-20. doi: 10.1016/j.micres.2010.10.003. Epub 2011 Jan 15.

Authors

Dandan Chen¹, Jianqun Lin, Yuanyuan Che, Xiangmei Liu, Jianqiang Lin

Affiliation

¹ State Key Lab of Microbial Technology, Shandong University, Jinan 250100, China.

PMID: 21239150
DOI: 10.1016/j.micres.2010.10.003

Abstract

A mercury-resistant plasmid of pTMJ212 which was able to shuttle between Acidithiobacillus caldus and Escherichia coli was constructed by inserting the mercury resistant determinants, the mer operon of Acidithiobacillus ferrooxidans, into the IncQ plasmid of pJRD215. pTMJ212 was transferred from Escherichia coli into Acidithiobacillus caldus through conjugation. Furthermore, pTMJ212 was transferred back from Acidithiobacillus caldus into Escherichia coli, thereby confirming the initial transfer of pTMJ212 from Escherichia coli to Acidithiobacillus caldus. Compared to the control, the cell growth of the recombinant Acidithiobacillus caldus increased markedly under mercury (Hg(2+)) stress especially at Hg(2+) concentrations ranging from 2.0 to 4.5 μg/ml.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acidithiobacillus / drug effects*
Acidithiobacillus / genetics*
Anti-Bacterial Agents / toxicity*
Conjugation, Genetic
Drug Resistance, Bacterial*
Escherichia coli / genetics
Gene Transfer, Horizontal
Genetic Vectors
Mercury / toxicity*
Microbial Sensitivity Tests
Plasmids

Substances

Anti-Bacterial Agents
Mercury