Abstract
Four biotinylated tri and tetrasaccharide fragments of plant and invertebrate N-glycans were synthesized using methyl tert-butyl phenyl (MBP) thioglycosides donors in order to evaluate their involvement in cross-allergies as cross-reactive carbohydrate determinants (CCDs). Various levels of reactivity to anti-bee and anti-HRP antibodies and with sera from allergic patients were observed when the conjugates were coated on streptavidin microplates. The results showed the potential utility of these xylosylated and fucosylated oligosaccharide fragments in determining CCD antibody epitopes.
Copyright © 2010 Elsevier Ltd. All rights reserved.
MeSH terms
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Allergens / immunology
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Antibodies / immunology*
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Bee Venoms / immunology
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Biotin / analogs & derivatives
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Biotin / chemical synthesis
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Biotin / chemistry
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Biotin / immunology
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Carbohydrate Sequence
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Cross Reactions*
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Epitopes / immunology
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Fucose / analogs & derivatives
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Fucose / immunology
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Horseradish Peroxidase / immunology
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Humans
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Hypersensitivity / diagnosis*
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Hypersensitivity, Immediate / diagnosis
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Immunoglobulin E / blood
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Immunoglobulin E / immunology
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Molecular Sequence Data
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Polysaccharides / chemical synthesis*
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Polysaccharides / immunology*
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Streptavidin
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Trisaccharides / chemical synthesis*
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Trisaccharides / chemistry
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Trisaccharides / immunology*
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Xylose / analogs & derivatives
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Xylose / immunology
Substances
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Allergens
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Antibodies
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Bee Venoms
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Epitopes
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Polysaccharides
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Trisaccharides
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Fucose
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Immunoglobulin E
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Biotin
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Streptavidin
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Xylose
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Horseradish Peroxidase