Abstract
In the past 25 years, there has been only one case of Japanese encephalitis in horses in Japan. We determined the full genome sequence of the Japanese encephalitis virus (JEV) strain JEV/eq/Tottori/2003 isolated from an afflicted horse and also analyzed its virulence in mice. The sequence analysis showed that the genome of JEV/eq/Tottori/2003 is similar to that of genotype I, a dominant genotype of JEV presently circulating in Japan. Its neurovirulence, but not neuroinvasiveness, was still as high as it was for genotype III, thus indicating the necessity for continuation of a vaccination program of horses against JEV.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Assay / veterinary
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Encephalitis Virus, Japanese / genetics*
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Encephalitis Virus, Japanese / pathogenicity
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Encephalitis, Japanese / veterinary*
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Encephalitis, Japanese / virology
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Genome, Viral
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Genotype
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Horse Diseases / virology*
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Horses
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Japan
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Phylogeny
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RNA, Viral / chemistry
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RNA, Viral / genetics
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Reverse Transcriptase Polymerase Chain Reaction / veterinary
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Viral Envelope Proteins / chemistry
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Viral Envelope Proteins / genetics
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Virulence
Substances
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RNA, Viral
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Viral Envelope Proteins