Abstract
The cytoplasmic domain of the medicinal mushroom Cordyceps militaris β-1,3-glucan synthase catalytic subunit Fks1 was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and was assayed for binding specificity. The recombinant cytoplasmic domain bound specifically to UDP-agarose and lichenan (β-glucan), but not to ADP-agarose, GDP-agarose, or other carbohydrates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Catalytic Domain*
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Cordyceps / cytology*
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Cordyceps / enzymology
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Cytoplasm / enzymology*
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Glucosyltransferases / chemistry*
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Glucosyltransferases / metabolism*
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Recombinant Proteins / chemistry*
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Recombinant Proteins / metabolism*
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Substrate Specificity
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Uridine Diphosphate / metabolism
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beta-Glucans / metabolism
Substances
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Recombinant Proteins
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beta-Glucans
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Uridine Diphosphate
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Glucosyltransferases
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1,3-beta-glucan synthase