Evaluation of serological diagnostic methods for the 2009 pandemic influenza A (H1N1) virus

Clin Vaccine Immunol. 2011 Mar;18(3):520-2. doi: 10.1128/CVI.00449-10. Epub 2011 Jan 12.

Abstract

Serology improves influenza diagnosis by capturing cases missed by reverse transcriptase PCR (RT-PCR). We prospectively evaluated microneutralization and hemagglutination inhibition assays for 2009 influenza A (H1N1) virus diagnosis among 24 RT-PCR-confirmed cases and 98 household contacts. Compared to hemagglutination inhibition, microneutralization demonstrated a higher level of concordance with RT-PCR (kappa = 0.69 versus kappa = 0.60) and greater sensitivity (83% versus 71%; P = 0.016).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Clinical Laboratory Techniques / methods*
  • Hemagglutination Inhibition Tests / methods
  • Humans
  • Influenza A Virus, H1N1 Subtype / immunology
  • Influenza A Virus, H1N1 Subtype / isolation & purification*
  • Influenza, Human / diagnosis*
  • Middle Aged
  • Neutralization Tests / methods
  • Prospective Studies
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sensitivity and Specificity
  • Serologic Tests / methods
  • Young Adult

Substances

  • RNA, Viral