Analysing cellulose biosynthesis with confocal microscopy

Abstract

Plant cells are delimited by a rigid cell wall that resists internal turgor pressure, but extends with a remarkable degree of control that allows the cell to grow and acquire specific shapes. Live cell fluorescence microscopy systems have allowed an amazing view into the complex and dynamic lives of individual proteins during cell morphogenesis. The current chapter will focus on methodology for live cell imaging of cellulose synthase (CESA) in Arabidopsis, which will also provide a launching pad to explore ones specific protein of interest.