Abstract
Antibodies to MOG in serum have a dubious prognostic value in multiple sclerosis. The MOG recombinant protein conformational properties relevant to the antigenic activity are unknown. We employed a solid-phase ELISA based on a product (rMOG(ED)(His)(6)) expressed in E. coli after subcloning the cDNA of the extracellular domain of rat MOG, performing a refolding procedure on column and affinity purification. The far-UV Circular Dichroism (CD) spectra of rMOG(ED)(His)(6) showed a β-sheet, a characteristic feature of the Ig-fold. However, in MS sera and controls we failed to detected IgM or IgG antibodies.
Copyright © 2010 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Extracellular Space / chemistry
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Extracellular Space / immunology
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Female
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Humans
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Immunoglobulin G / analysis
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Immunoglobulin G / blood*
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Immunoglobulin M / analysis
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Immunoglobulin M / blood*
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Male
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Middle Aged
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Multiple Sclerosis / diagnosis
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Multiple Sclerosis / immunology*
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Multiple Sclerosis / metabolism*
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Myelin Proteins
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Myelin-Associated Glycoprotein / chemistry
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Myelin-Associated Glycoprotein / immunology*
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Myelin-Oligodendrocyte Glycoprotein
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Protein Folding*
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Protein Structure, Tertiary / physiology
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Rats
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Young Adult
Substances
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Immunoglobulin G
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Immunoglobulin M
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MOG protein, human
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Mog protein, rat
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Myelin Proteins
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Myelin-Associated Glycoprotein
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Myelin-Oligodendrocyte Glycoprotein
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Recombinant Proteins