Temporal evolution of microbiological, physical, and chemical quality of stormwater runoff from a stormwater drain in an urban residential area in Calgary, Canada, was investigated from May to September, 2006 and 2007. Investigating event mean concentrations and their correlations with rainfall characteristics revealed that intensive rainfall events produced highly polluted stormwater runoff when pollutant source limitation did not occur. Inconsistent event-based correlations between total suspended solids (TSS) concentrations and water quality parameters were observed. During storms, the loading of TSS exhibited a flow-dependent nature, whereas microorganism discharge appeared to be governed by a flow-independent mechanism. No strong first-flush effect was observed in either TSS or microorganisms, on average. No correlations of first-flush loads of TSS with rainfall characteristics were identified. Moderate negative correlations between first-flush loads of microorganisms and rainfall depth and intensity indicated that first flush of microorganisms tended to occur in small storms.