Abstract
Development of autologous scaffolds has been highly desired for implantation without eliciting adverse inflammatory and immune responses. However, it has been difficult to obtain autologous scaffolds by tissue decellularization because of the restricted availability of autologous donor tissues from a patient. Here we report a method to prepare autologous extracellular matrix (aECM) scaffolds by combining culture of autologous cells in a three-dimensional template, decellularization, and template removal. The aECM scaffolds showed excellent biocompatibility when implanted. We anticipate that "Full Autologous Tissue Engineering" can be realized to minimize undesirable host tissue responses by culturing the patient's own cells in an aECM scaffold.
Copyright © 2010 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigen-Presenting Cells / cytology
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Antigen-Presenting Cells / drug effects
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Antigen-Presenting Cells / metabolism
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DNA / metabolism
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Extracellular Matrix / drug effects
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Extracellular Matrix / metabolism*
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Extracellular Matrix / ultrastructure
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Fluorescent Antibody Technique
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Gene Expression Regulation / drug effects
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Glycosaminoglycans / metabolism
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Humans
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Interleukin-10 / genetics
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Interleukin-10 / metabolism
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Lactic Acid / pharmacology
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / drug effects
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Mesenchymal Stem Cells / ultrastructure
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Mice
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Microscopy, Fluorescence
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Neutrophils / cytology
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Neutrophils / drug effects
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Neutrophils / metabolism
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Polyglycolic Acid / pharmacology
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Polylactic Acid-Polyglycolic Acid Copolymer
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Solubility / drug effects
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Tissue Engineering / methods*
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Tissue Scaffolds / chemistry*
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Tumor Necrosis Factor-alpha / genetics
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Glycosaminoglycans
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Tumor Necrosis Factor-alpha
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Interleukin-10
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Polylactic Acid-Polyglycolic Acid Copolymer
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Polyglycolic Acid
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Lactic Acid
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DNA