Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests

C H Carbonetto; E L Malchiodi; M Chiaramonte; E Durante de Isola; C A Fossati; R A Margni

doi:10.1111/j.1365-2249.1990.tb05409.x

Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests

Clin Exp Immunol. 1990 Oct;82(1):93-6. doi: 10.1111/j.1365-2249.1990.tb05409.x.

Authors

C H Carbonetto¹, E L Malchiodi, M Chiaramonte, E Durante de Isola, C A Fossati, R A Margni

Affiliation

¹ Institute of Humoral Immunity Studies (IDEHU) CONICET/UBA, Department of Immunology, Faculty of Pharmacology and Biochemistry, Argentina.

Abstract

By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas' disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / immunology*
Antigens, Protozoan / immunology
Antigens, Protozoan / isolation & purification*
Chagas Disease / blood
Chagas Disease / diagnosis*
Chromatography, Affinity / methods*
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin Isotypes
Leishmaniasis / blood
Mice
Mice, Inbred BALB C
Trypanosoma cruzi / immunology*

Substances

Antibodies, Monoclonal
Antigens, Protozoan
Immunoglobulin Isotypes