A yellow-pigmented, Gram-staining-negative, non-motile, strictly aerobic and rod-shaped bacterium, designated CS100(T), was isolated from soil in Chungbuk, Korea. Phylogenetic analysis and comparative studies based on the 16S rRNA gene sequence showed that strain CS100(T) belonged to the genus Flavobacterium in the family Flavobacteriaceae. Strain CS100(T) showed the highest sequence similarities to Flavobacterium glaciei JCM 13953(T) (97.6 %) and Flavobacterium johnsoniae KACC 11410(T) (97.1 %). Sequence similarity to other members of the genus Flavobacterium was 91.5-97.0 %. Growth occurred at 4-30 °C, at pH 5.0-9.0 and in the presence of 0-2 % (w/v) NaCl. Flexirubin-type pigments were produced. Menaquinone-6 (MK-6) was the major respiratory quinone and the major fatty acids were iso-C(15 : 0) (17.3 %), summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)ω7c, 15.5 %) and C(16 : 0) (11.8 %). The DNA G+C content was 36.4 mol%. Strain CS100(T) hydrolysed skimmed milk and gelatin, but not chitin or pectin, and showed oxidase and catalase activities. DNA-DNA relatedness was 3.0 % with F. glaciei JCM 13953(T) and 11.5 % with F. johnsoniae KACC 11410(T). On the basis of the evidence from this study, strain CS100(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium chungbukense sp. nov. is proposed. The type strain is CS100(T) ( = KACC 15048(T) = JCM 17386(T)).