Abstract
Zosima absinthifolia (ZA) extracts and the coumarins (+)-columbianadin and (-)-deltoin were evaluated for their potential hepatoprotective and antiinflamatory effects in a CCl(4)-induced hepatotoxicity assay in rats and by the inhibition of TNF-α production on LPS-stimulated THP-1 macrophages, respectively. Both the ZA extracts and the coumarins showed hepatoprotective activity confirmed by monitoring the ALT/AST levels and by histopathological examination. The antiinflamatory activity, proved by the inhibition of TNF-α production, was found to be higher for the n-hexane root extract than for coumarins, suggesting synergic potential of the extract. The concentration of (-)-deltoin and (+)-columbianadin in extracts was determined by HPLC analysis.
Copyright © 2010 Elsevier B.V. All rights reserved.
MeSH terms
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Alanine Transaminase / metabolism
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Animals
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Anti-Inflammatory Agents / analysis
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Anti-Inflammatory Agents / pharmacology
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Anti-Inflammatory Agents / therapeutic use*
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Apiaceae / chemistry*
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Aspartate Aminotransferases / metabolism
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Carbon Tetrachloride
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Cell Line
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Chemical and Drug Induced Liver Injury / drug therapy*
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Chemical and Drug Induced Liver Injury / enzymology
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Chemical and Drug Induced Liver Injury / pathology
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Coumarins / analysis
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Coumarins / pharmacology
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Coumarins / therapeutic use*
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Drug Synergism
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Female
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Lipopolysaccharides
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Liver / drug effects*
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Liver / enzymology
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Liver / pathology
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Macrophages / drug effects
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Male
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Phytotherapy*
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use
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Plant Roots
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Rats
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Rats, Sprague-Dawley
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Tumor Necrosis Factor-alpha / biosynthesis*
Substances
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Anti-Inflammatory Agents
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Coumarins
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Lipopolysaccharides
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Plant Extracts
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Tumor Necrosis Factor-alpha
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Carbon Tetrachloride
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Aspartate Aminotransferases
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Alanine Transaminase