Abstract
We use a powerful method to replace wild-type genes on the chromosome of Escherichia coli. Using a unique form of PCR, we generate easily constructible gene fusions bearing single point mutations. Used in conjunction with homologous recombination, this method eliminates cloning procedures previously used for this purpose.
Copyright © 2010 Elsevier B.V. All rights reserved.
MeSH terms
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Artificial Gene Fusion
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Chromosome Mapping / methods*
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Chromosomes, Bacterial
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Escherichia coli / genetics*
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Escherichia coli Proteins / genetics*
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Genes, Bacterial*
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Genetics, Microbial / methods*
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Molecular Biology / methods
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Mutant Proteins / genetics*
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Point Mutation*
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Polymerase Chain Reaction / methods
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Recombination, Genetic
Substances
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Escherichia coli Proteins
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Mutant Proteins