Aim: To study the effect of promoting aldosterone on proliferation of ventricular fibroblasts.
Methods: Assay of [3H]-TdR incorporation rate and RT-PCR were used.
Results: Aldosterone could promote [3H]-TdR incorporation of ventricular fibroblasts, the effective dose of aldosterone was among (1 x 10(-9)-1 x 10(-6))mol/L, and had dose-dependent manner, the c-fos gene was expressed after stimulated by aldosterone for 15 min, and studied the highest in 1 h, then reduced later. Spironolactone, aldosterone receptor antagonist could block the effect of aldosterone.
Conclusion: Aldosterone promotes the proliferation of ventricular fibroblasts, mediated by aldosterone receptor.