A method was developed for the determination of 153 pesticide residues in tea using on-line gel permeation chromatography-gas chromatography/mass spectrometry (GPC-GC/ MS). The pesticide residues were extracted with acetonitrile under ultrasonic operation, and the extract was first cleaned up with an ENVI-carb solid phase extraction column and then separated and detected with the on-line GPC-GC/MS system. The recoveries of the method ranged from 73.32% to 117.05% with the relative standard deviations (RSDs) from 0.76% to 13.18%. The limits of detection and the limits of quantification were 0.0003-0.006 mg/kg and 0.001-0.02 mg/kg, respectively. This method is simple, rapid and characterized with acceptable sensitivity and accuracy to meet the requirements for the analysis of multiple pesticide residues in tea.