Structure of the inhibitor complex of old yellow enzyme from Trypanosoma cruzi

Keishi Yamaguchi; Naoki Okamoto; Keiji Tokuoka; Shigeru Sugiyama; Nahoko Uchiyama; Hiroyoshi Matsumura; Koji Inaka; Yoshihiro Urade; Tsuyoshi Inoue

doi:10.1107/S0909049510033595

Structure of the inhibitor complex of old yellow enzyme from Trypanosoma cruzi

J Synchrotron Radiat. 2011 Jan;18(1):66-9. doi: 10.1107/S0909049510033595. Epub 2010 Nov 12.

Authors

Keishi Yamaguchi¹, Naoki Okamoto, Keiji Tokuoka, Shigeru Sugiyama, Nahoko Uchiyama, Hiroyoshi Matsumura, Koji Inaka, Yoshihiro Urade, Tsuyoshi Inoue

Affiliation

¹ Department of Applied Chemistry, Graduate School of Engineering, Osaka University, Yamada-Oka 2-1, Suita, Osaka 565-0871, Japan.

Abstract

Old yellow enzyme (OYE) is an NADPH oxidoreductase which contains flavin mononucleotide as prosthetic group. The X-ray structures of OYE from Trypanosoma cruzi (TcOYE) which produces prostaglandin (PG) F(2α) from PGH(2) have been determined in the presence or absence of menadione. The binding motif of menadione, known as one of the inhibitors for TcOYE, should accelerate the structure-based development of novel anti-chagasic drugs that inhibit PGF(2α) production specifically.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Crystallography, X-Ray
Flavin Mononucleotide / chemistry
Hydroxyprostaglandin Dehydrogenases / antagonists & inhibitors*
Hydroxyprostaglandin Dehydrogenases / metabolism
Models, Molecular
NADPH Dehydrogenase / antagonists & inhibitors*
NADPH Dehydrogenase / chemistry*
Trypanosoma cruzi / enzymology*
Vitamin K 3 / chemistry*
Vitamin K 3 / pharmacology

Substances

Vitamin K 3
Flavin Mononucleotide
Hydroxyprostaglandin Dehydrogenases
prostaglandin-F synthase
NADPH Dehydrogenase