CYP450 biosensors based on screen-printed carbon electrodes for the determination of cocaine

Laura Asturias-Arribas; M Asunción Alonso-Lomillo; Olga Domínguez-Renedo; M Julia Arcos-Martínez

doi:10.1016/j.aca.2010.11.006

CYP450 biosensors based on screen-printed carbon electrodes for the determination of cocaine

Anal Chim Acta. 2011 Jan 24;685(1):15-20. doi: 10.1016/j.aca.2010.11.006. Epub 2010 Nov 10.

Authors

Laura Asturias-Arribas¹, M Asunción Alonso-Lomillo, Olga Domínguez-Renedo, M Julia Arcos-Martínez

Affiliation

¹ Analytical Chemistry Department, Faculty of Sciences, University of Burgos, Pza. Misael Bañuelos s/n, 09001 Burgos, Spain.

PMID: 21168546
DOI: 10.1016/j.aca.2010.11.006

Abstract

A new electrochemical method has been described and characterized for the determination of cocaine using screen-printed biosensors. The enzyme cytochrome P450 was covalently attached to screen-printed carbon electrodes. Experimental design methodology has been performed to optimize the pH and the applied potential, both variables that have an influence on the chronoamperometric determination of the drug. This method showed a reproducibility of 3.56% (n=4) related to the slopes of the calibration curves performed in the range from 19 up to 166nM. It has been probed the used of this kind of biosensors in the determination of cocaine in street samples, with an average capability of detection of 23.05±3.53nM (n=3, α=β=0.05).

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Aryl Hydrocarbon Hydroxylases / metabolism*
Biosensing Techniques / methods*
Carbon / chemistry
Crack Cocaine / analysis*
Crack Cocaine / metabolism
Cytochrome P450 Family 2
Electrochemistry / methods
Enzymes, Immobilized / metabolism
Reproducibility of Results
Sensitivity and Specificity

Substances

Crack Cocaine
Enzymes, Immobilized
Carbon
Aryl Hydrocarbon Hydroxylases
Cytochrome P450 Family 2
cytochrome P-450 CYP2B4 (rabbit)