Preservation of viable Francisella tularensis for forensic analysis

Nancy B Valentine; Sharon C Wunschel; Catherine O Valdez; Helen Kreuzer; Rachel A Bartholomew; Timothy M Straub; Karen L Wahl

doi:10.1016/j.mimet.2010.12.006

Preservation of viable Francisella tularensis for forensic analysis

J Microbiol Methods. 2011 Feb;84(2):346-8. doi: 10.1016/j.mimet.2010.12.006. Epub 2010 Dec 16.

Authors

Nancy B Valentine¹, Sharon C Wunschel, Catherine O Valdez, Helen Kreuzer, Rachel A Bartholomew, Timothy M Straub, Karen L Wahl

Affiliation

¹ Pacific Northwest National Laboratory, Richland, WA 99352, USA. nancy.valentine@pnl.gov

PMID: 21167882
DOI: 10.1016/j.mimet.2010.12.006

Abstract

As a preservation solution, (1%) ammonium chloride may be preferred over other conventionally used storage solutions because of its compatibility with analytical techniques such as Mass Spectrometry. In this study, ammonium chloride performed as well or better than phosphate buffered saline with Tween or Butterfields/Tween for preserving Francisella tularensis subsp. novicida.

Publication types

Comparative Study
Evaluation Study
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Ammonium Chloride / metabolism
Forensic Medicine / methods*
Francisella tularensis / isolation & purification*
Humans
Microbial Viability*
Preservation, Biological / methods*
Specimen Handling / methods*

Substances

Ammonium Chloride