[Distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes]

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 Feb;21(1):112-4.
[Article in Chinese]

Abstract

Aim: To study the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes.

Methods: The neonatal rat cardiomyocytes were cultured. According to the different grow period of neonatal rat cardiomyocytes, the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes were studied.

Results: The efficiency was significantly increased after pEGFP-N1 plasmid transfection in one-day cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.

Conclusion: The efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes was related to the grow period of neonatal rat cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Cells, Cultured
  • Green Fluorescent Proteins / genetics*
  • Green Fluorescent Proteins / metabolism*
  • Myocytes, Cardiac / metabolism*
  • Plasmids
  • Rats
  • Rats, Wistar
  • Transfection*

Substances

  • Green Fluorescent Proteins