Production of arachidonic and linoleic acid metabolites by guinea pig tracheal epithelial cells

Inflammation. 1990 Aug;14(4):401-8. doi: 10.1007/BF00914091.

Abstract

Pulmonary epithelial cells may be responsible for regulating airway smooth muscle function, in part by release of fatty acid-derived mediators. Incubation of isolated guinea pig tracheal epithelial cells with radiolabeled arachidonic acid (AA) leads to the production of 5- and 15-hydroxyeicosatetraenoic acid (5- and 15-HETE) and smaller amounts of leukotriene (LT) B4 and C4 and 12-hydroxyheptadecatrienoic acid (HHT). Epithelial cells also are able to release linoleic acid (LA) metabolites. Incubation with radiolabeled linoleic acid leads to the formation of 9- and 13-hydroxyoctadecadienoic acid (9- and 13-HODE). The biological significance of these mediators produced by epithelial cells is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antithrombins / biosynthesis
  • Arachidonic Acid
  • Arachidonic Acids / metabolism*
  • Carbon Radioisotopes
  • Epithelial Cells
  • Epithelium / metabolism
  • Fatty Acids / metabolism
  • Guinea Pigs
  • Hydroxyeicosatetraenoic Acids / biosynthesis
  • Linoleic Acids / biosynthesis
  • Linoleic Acids / metabolism*
  • Linoleic Acids, Conjugated*
  • Male
  • Perfusion
  • Trachea / metabolism*

Substances

  • Antithrombins
  • Arachidonic Acids
  • Carbon Radioisotopes
  • Fatty Acids
  • Hydroxyeicosatetraenoic Acids
  • Linoleic Acids
  • Linoleic Acids, Conjugated
  • 9-hydroxy-10,12-octadecadienoic acid
  • Arachidonic Acid
  • 5-hydroxy-6,8,11,14-eicosatetraenoic acid
  • 13-hydroxy-9,11-octadecadienoic acid
  • 15-hydroxy-5,8,11,13-eicosatetraenoic acid