Background and objective: precious studies proven that the overexpression of eukaryotic translation initiation factor 4E (eIF4E) in a variety of solid tumors has linked with malignant tumorigenesis, invasion and metastasis. The aim of this work is to explore the expression of eIF4E and its clinical significance in non-small cell lung cancer (NSCLC) through immunohistochemical method.
Methods: seventy NSCLC specimens were constructed into tissue microarray, simultaneously took 19 cases adjacent tissues and 20 cases normal lung tissue as control, all performed using immunohistochemistry (Envision).
Results: eIF4E expression in NSCLC was significantly higher than that in adjacent tissues and normal lung tissues, statistically significant difference between the groups. The eIF4E positive expression in lymph node metastasis group was significantly higher than that in no-lymph node metastasis. eIF4E protein positive expression of WD/MD cancer tissue was significantly lower than those in PD/ND cancer tissue. There were no significant differences of positive expression of eIF4E with the tumor diameter, age, smoking status, gender of NSCLC patients (P > 0.05).
Conclusions: there were highly over expression rate of eIF4E, and closely correlated with lymphnode metastasis in NSCLC, which implies that the eIF4E expression may be related to tumorgenesis, invasion and metastasis of lung cancer. eIF4E may be a new marker for lung cancer and objective indicators assessing the development of lung cancer.
背景与目的: 以往研究表明，真核细胞翻译起始因子（eukaryotic initiation factor-4E, eIF4E）在多种实体肿瘤中高表达，与恶性肿瘤发生、浸润和转移密切相关。本研究旨在采用免疫组化方法研究eIF4E在非小细胞肺癌（non-small cell lung cancer, NSCLC）中的表达及意义。
方法: 70例NSCLC石蜡标本制成组织芯片，以19例癌旁组织、20例正常肺组织为对照，均采用Envision两步法进行免疫组化检测。
结果: eIF4E在NSCLC中的阳性表达率高于在癌旁组织和正常肺组织的表达率；eIF4E在淋巴结转移组阳性表达率高于在无淋巴结转移组中表达率；eIF4E在低分化组中阳性表达率高于在中高分化组中表达率。eIF4E阳性表达与患者性别、年龄、肿块大小、吸烟情况无统计学差异（P > 0.05）。
结论: eIF4E在NSCLC中有较高的表达率，且与淋巴结转移密切相关，提示NSCLC的发生、侵袭和转移可能与eIF4E相关，eIF4E有可能作为肺癌新的标志物以及评估肺癌进展客观指标。