Aim: To explore the influence of cold strss on DNA oxidative damage of lung in chicken.
Methods: Took 15-day-old healthy chicks as the experimental object, carried on the cold stress (12 +/- 1 degrees C) to process. Detected the change of the MDA content, SOD and GSH-Px activity of the lung, and performed KCl-SDS precipitation method and fluorescence detection method to identify the influence of cold strss on DNA-protein crosslinks (DPC) and DNA-DNA crosslinks (DDC) of lung cell in different time.
Results: The results were as follow: with the time lapsing during acute cold stress, MDA content gradually increased, the SOD and GSH-Px activity of the lung increased compared with their control group at each stress time point, and the lung cell DPC and DDC coefficient were all gradually increased with the time lapsing.
Conclusion: Cold stress could bring about destruction in the lung tissue oxidation-antioxidant balance, and causes the oxidation damage of DNA.