Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor

Nat Struct Mol Biol. 2011 Jan;18(1):88-90. doi: 10.1038/nsmb.1952. Epub 2010 Dec 12.

Abstract

We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / metabolism
  • Cryoelectron Microscopy
  • Escherichia coli / genetics*
  • Escherichia coli Proteins / chemistry*
  • Escherichia coli Proteins / metabolism
  • Models, Molecular
  • Protein Biosynthesis
  • Protein Structure, Tertiary
  • Receptors, Cytoplasmic and Nuclear / chemistry*
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Receptors, Peptide / chemistry*
  • Ribosomes / chemistry
  • Ribosomes / ultrastructure*
  • Signal Recognition Particle / chemistry*
  • Signal Recognition Particle / metabolism

Substances

  • Bacterial Proteins
  • Escherichia coli Proteins
  • FtsY protein, Bacteria
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Peptide
  • Signal Recognition Particle
  • signal peptide receptor

Associated data

  • PDB/2XKV
  • PDB/EMD-1762