Effect on the epigallocatechin gallate/epigallocatechin ratio in a green tea (Camellia sinensis L.) extract of different extraction temperatures and its effect on IgA production in mice

Biosci Biotechnol Biochem. 2010;74(12):2501-3. doi: 10.1271/bbb.100498. Epub 2010 Dec 7.

Abstract

We found that the epigallocatechin gallate (EGCG)/epigallocatechin (EGC) ratio in a green tea (Camellia sinensis L.) extract was affected by the extraction temperature. The EGCG/EGC ratio in the 4 °C extract was around 1:3-4, whereas in the 100 °C extract, it was around 1:0.7. Oral administration of the mixture with a high EGC ratio (1:2-3 = EGCG/EGC) resulted in greater IgA production by murine Peyer's patch cells.

MeSH terms

  • Animals
  • Camellia sinensis / chemistry*
  • Catechin / analogs & derivatives
  • Catechin / analysis*
  • Catechin / isolation & purification
  • Catechin / pharmacology*
  • Chemical Fractionation / methods*
  • Female
  • Immunoglobulin A / immunology*
  • Immunologic Factors / analysis
  • Immunologic Factors / isolation & purification
  • Immunologic Factors / pharmacology
  • Mice
  • Mice, Inbred BALB C
  • Peyer's Patches / cytology
  • Peyer's Patches / drug effects
  • Peyer's Patches / immunology
  • Tea / chemistry*
  • Temperature*

Substances

  • Immunoglobulin A
  • Immunologic Factors
  • Tea
  • Catechin
  • epigallocatechin gallate
  • gallocatechol