Abstract
From seeds of Nigella sativa L. (Ranunculaceae), an endemic plant of Uzbekistan, two novel defensins named Ns-D1 and Ns-D2, were isolated and sequenced. The peptides differ by a single amino acid residue and show high sequence similarity to Raphanus sativus L. defensins Rs-AFP1 and Rs-AFP2. The Ns-D1 and Ns-D2 defensins display strong although divergent antifungal activity towards a number of phytopathogenic fungi. High antifungal activity of N. sativa defensins makes them promising candidates for engineering pathogen-resistant plants.
Copyright © 2010 Elsevier Masson SAS. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Anti-Bacterial Agents / chemistry*
-
Anti-Bacterial Agents / isolation & purification
-
Anti-Bacterial Agents / pharmacology
-
Antifungal Agents / chemistry*
-
Antifungal Agents / isolation & purification
-
Antifungal Agents / pharmacology
-
Chromatography, Ion Exchange
-
Defensins / chemistry*
-
Defensins / isolation & purification
-
Defensins / pharmacology
-
Microbial Sensitivity Tests
-
Molecular Sequence Data
-
Nigella sativa / chemistry*
-
Seeds / chemistry*
-
Sequence Homology, Amino Acid
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
-
Anti-Bacterial Agents
-
Antifungal Agents
-
Defensins