The aim of this study was to analyse tropism evolution in HIV-1 patients with at least 2 years of viral load undetectability. For this purpose, HIV-1-infected antiretroviral-treated patients (n = 128) with a viral load less than 50 copies/ml for 4 years in median were studied. No change of virus tropism was observed in 92.9% of patients. Therefore, before the use of CCR5 antagonists, genotypic viral DNA tropism determination is an available tool.