Silencing of the gene for homeobox protein HOXB13 by siRNA confers resistance to methylmercury on HEK293 cells

Gi-Wook Hwang; Katsunori Ryoke; Tsutomu Takahashi; Akira Naganuma

doi:10.2131/jts.35.941

Silencing of the gene for homeobox protein HOXB13 by siRNA confers resistance to methylmercury on HEK293 cells

J Toxicol Sci. 2010 Dec;35(6):941-4. doi: 10.2131/jts.35.941.

Authors

Gi-Wook Hwang¹, Katsunori Ryoke, Tsutomu Takahashi, Akira Naganuma

Affiliation

¹ Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.

PMID: 21139345
DOI: 10.2131/jts.35.941

Abstract

Methylmercury is an environmental pollutant that causes severe central nervous system disorders. We searched for transcription factors involved in the development of methylmercury toxicity and found that the decreased expression of a homeobox protein, HOXB13, in HEK293 cells conferred strong resistance to methylmercury.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques
Cell Survival / drug effects
Cell Survival / genetics
Drug Resistance / genetics*
Environmental Pollutants / toxicity*
Gene Silencing*
HEK293 Cells
Homeodomain Proteins / genetics*
Humans
Methylmercury Compounds / toxicity*
RNA, Small Interfering / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Transfection

Substances

Environmental Pollutants
HOXB13 protein, human
Homeodomain Proteins
Methylmercury Compounds
RNA, Small Interfering
methylmercuric chloride