[Study on purification of total flavonoids and saponins of Astragalus with macroporous resin]

Zhong Yao Cai. 2010 Jul;33(7):1163-6.
[Article in Chinese]

Abstract

Objective: Through systematic study on many factors of influence of macroporous resin adsorption and separation, screening the best macroporous resin for purification of total flavonoids and saponins of Astragalus.

Methods: The adsorption and desorption properties of total flavonoids and saponins on macroporous resins including D101, AB-8, XAD-1, HP-20, SP825, LSA-40, LX-18, XDA-5 and ADS-7 were compared. According to the results, XDA-5, D101 and AB-8 offered higher adsorption and desorption capacities and higher adsorption speed for total flavonoids and saponins than other resins. Column packed with XDA-5 resin was used to perform dynamic adsorption and desorption tests to optimize the separation process.

Results: XDA-5 type macroporous resin owned optimum adsorption and elution parameters. The dynamic adsorption ratio of total flavonoids of Astragalus was 58.83 mg/mL after eluted with 4BV of distilled water and 4BV of 30%, 50%, 70% ethanol, respectively. Contents of total flavonoids and saponins of Astragalus were 28.75%, 44.38%, respectively.

Conclusion: It is a reliable and accurate method for development and industrial production of compound drug containing Astragalus.

Publication types

  • English Abstract

MeSH terms

  • Adsorption
  • Astragalus Plant / chemistry*
  • Flavonoids / analysis
  • Flavonoids / isolation & purification*
  • Plant Roots / chemistry
  • Plants, Medicinal / chemistry
  • Resins, Synthetic / chemistry*
  • Saponins / analysis
  • Saponins / isolation & purification*
  • Solvents / chemistry
  • Spectrophotometry, Ultraviolet
  • Technology, Pharmaceutical / methods*
  • Water / chemistry

Substances

  • Flavonoids
  • Resins, Synthetic
  • Saponins
  • Solvents
  • Water