Abstract
We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli. This, in turn, allows the isolation of highly pure FMN.
MeSH terms
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Biotechnology / methods
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Coenzymes / analysis
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Coenzymes / isolation & purification*
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Coenzymes / metabolism*
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Escherichia coli / growth & development
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Escherichia coli / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Flavin Mononucleotide / analysis
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Flavin Mononucleotide / isolation & purification*
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Flavin Mononucleotide / metabolism*
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Flavoproteins / chemistry
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Flavoproteins / metabolism*
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Gene Expression Regulation, Bacterial
Substances
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Coenzymes
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Escherichia coli Proteins
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Flavoproteins
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Flavin Mononucleotide