Identification of a tyrosine decarboxylase gene (tdcA) in Streptococcus thermophilus 1TT45 and analysis of its expression and tyramine production in milk

Appl Environ Microbiol. 2011 Feb;77(3):1140-4. doi: 10.1128/AEM.01928-10. Epub 2010 Dec 3.

Abstract

In this study, a tyrosine decarboxylase gene (tdcA) was identified in 1 among 83 Streptococcus thermophilus strains tested. Its sequence, nearly identical to that of a tdcA of Lactobacillus curvatus, indicated a horizontal gene transfer event. Transcription in milk and the formation of critical levels of tyramine were observed in the presence of tyrosine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Cheese / microbiology
  • Dairying
  • Milk / chemistry*
  • Milk / metabolism
  • Molecular Sequence Data
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Streptococcus thermophilus / enzymology*
  • Streptococcus thermophilus / genetics
  • Streptococcus thermophilus / metabolism
  • Tyramine / biosynthesis*
  • Tyrosine Decarboxylase / genetics*
  • Tyrosine Decarboxylase / metabolism*

Substances

  • Bacterial Proteins
  • Tyrosine Decarboxylase
  • Tyramine

Associated data

  • GENBANK/FR682467