Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

J Clin Microbiol. 2011 Feb;49(2):732-4. doi: 10.1128/JCM.01970-10. Epub 2010 Dec 1.

Abstract

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Typing Techniques / methods*
  • DNA Restriction-Modification Enzymes / genetics*
  • Humans
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Staphylococcal Infections / microbiology*
  • Staphylococcus aureus / classification*
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / isolation & purification*

Substances

  • DNA Restriction-Modification Enzymes