The β-adrenoceptor agonist isoproterenol promotes the activity of respiratory chain complex I and lowers cellular reactive oxygen species in fibroblasts and heart myoblasts

Eur J Pharmacol. 2011 Feb 10;652(1-3):15-22. doi: 10.1016/j.ejphar.2010.11.016. Epub 2010 Nov 29.

Abstract

A study is presented on the effect of the β-adrenoceptor agonist isoproterenol on mitochondrial oxygen metabolism in fibroblast and heart myoblast cultures. Isoproterenol treatment of serum-limited fibroblasts and proliferating myoblasts results in the promotion of mitochondrial complex I activity and decrease of the cellular level of reactive oxygen species. These effects of isoproterenol are associated with cAMP-dependent phosphorylation of complex I subunit(s). Addition of okadaic acid, inhibitor of protein phosphatase(s), reverses the decline of complex I activity in serum-limited fibroblast cultures and activates the complex in proliferating myoblast cultures. The effects of isoproterenol on complex I activity and reactive oxygen species balance can contribute to the therapeutic effect of the drug.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic beta-Agonists / pharmacology*
  • Cells, Cultured
  • Cyclic AMP / metabolism
  • Electron Transport Complex I / metabolism*
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Humans
  • Infant, Newborn
  • Isoproterenol / pharmacology*
  • Mitochondria / metabolism
  • Myoblasts, Cardiac / cytology
  • Myoblasts, Cardiac / drug effects*
  • Myoblasts, Cardiac / metabolism
  • Phosphorylation
  • Reactive Oxygen Species / metabolism*

Substances

  • Adrenergic beta-Agonists
  • Reactive Oxygen Species
  • Cyclic AMP
  • Electron Transport Complex I
  • Isoproterenol