Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function

Biochem Soc Trans. 2010 Dec;38(6):1655-9. doi: 10.1042/BST0381655.

Abstract

The CPC [chromosomal passenger complex; INCENP (inner centromere protein), Aurora B kinase, survivin and borealin] is implicated in many mitotic processes. In the present paper we describe how we generated DT40 conditional-knockout cell lines for incenp1 and survivin1 to better understand the role of these CPC subunits in the control of Aurora B kinase activity. These lines enabled us to reassess current knowledge of survivin function and to show that INCENP acts as a rheostat for Aurora B activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Aurora Kinase B
  • Aurora Kinases
  • Cell Line*
  • Chickens
  • Chromosomal Proteins, Non-Histone / genetics
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Chromosomes / metabolism
  • Gene Knockdown Techniques*
  • Humans
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Mitosis
  • Protein Serine-Threonine Kinases / metabolism*

Substances

  • Chromosomal Proteins, Non-Histone
  • Microtubule-Associated Proteins
  • AURKB protein, human
  • Aurora Kinase B
  • Aurora Kinases
  • Protein Serine-Threonine Kinases