Abstract
This chapter describes a method for monitoring nanoparticle treated human hepatocarcinoma cells (Hep G2) for apoptosis. The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Apoptosis / drug effects*
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Carcinoma, Hepatocellular / pathology*
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Caspase 3 / metabolism
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Cell Death / drug effects
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Cell Line, Tumor
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Coumarins / analysis
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Coumarins / metabolism
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Enzyme Activation / drug effects
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Humans
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Liver Neoplasms / pathology*
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Nanoparticles / toxicity*
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Oligopeptides / metabolism
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Spectrometry, Fluorescence
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Substrate Specificity
Substances
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Coumarins
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Oligopeptides
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aspartyl-glutamyl-valyl-aspartyl-7-amino-4-trifluoromethylcoumarin
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7-amino-4-trifluoromethylcoumarin
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Caspase 3