Monitoring nanoparticle-treated hepatocarcinoma cells for apoptosis

Methods Mol Biol. 2011:697:167-72. doi: 10.1007/978-1-60327-198-1_17.

Abstract

This chapter describes a method for monitoring nanoparticle treated human hepatocarcinoma cells (Hep G2) for apoptosis. The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Apoptosis / drug effects*
  • Carcinoma, Hepatocellular / pathology*
  • Caspase 3 / metabolism
  • Cell Death / drug effects
  • Cell Line, Tumor
  • Coumarins / analysis
  • Coumarins / metabolism
  • Enzyme Activation / drug effects
  • Humans
  • Liver Neoplasms / pathology*
  • Nanoparticles / toxicity*
  • Oligopeptides / metabolism
  • Spectrometry, Fluorescence
  • Substrate Specificity

Substances

  • Coumarins
  • Oligopeptides
  • aspartyl-glutamyl-valyl-aspartyl-7-amino-4-trifluoromethylcoumarin
  • 7-amino-4-trifluoromethylcoumarin
  • Caspase 3