Synthesis of histone H3 proteins by a thioacid capture ligation strategy

Chem Commun (Camb). 2011 Feb 14;47(6):1746-8. doi: 10.1039/c0cc03666h. Epub 2010 Nov 29.

Abstract

The first application of thioacid capture ligation in protein synthesis is described. Two histone H3 proteins were synthesized in which a 30 min ligation reaction gave the protein products in good yields.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid
  • Dithiothreitol / chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Histones / chemical synthesis*
  • Histones / chemistry
  • Indicators and Reagents / chemistry
  • Oxidation-Reduction
  • Phosphines / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sulfhydryl Compounds / chemistry*
  • Time Factors

Substances

  • Histones
  • Indicators and Reagents
  • Phosphines
  • Sulfhydryl Compounds
  • tris(2-carboxyethyl)phosphine
  • Dithiothreitol