Abstract
The first application of thioacid capture ligation in protein synthesis is described. Two histone H3 proteins were synthesized in which a 30 min ligation reaction gave the protein products in good yields.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatography, High Pressure Liquid
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Dithiothreitol / chemistry
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Electrophoresis, Polyacrylamide Gel
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Histones / chemical synthesis*
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Histones / chemistry
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Indicators and Reagents / chemistry
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Oxidation-Reduction
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Phosphines / chemistry
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Sulfhydryl Compounds / chemistry*
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Time Factors
Substances
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Histones
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Indicators and Reagents
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Phosphines
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Sulfhydryl Compounds
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tris(2-carboxyethyl)phosphine
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Dithiothreitol