Role of reactive oxygen species and Bcl-2 family proteins in TNF-α-induced apoptosis of lymphocytes

N V Ryazanceva; V V Novickiy; O B Zhukova; A K Biktasova; O E Chechina; E V Sazonova; M V Belkina; N Yu Chasovskih; Z K Khaitova

doi:10.1007/s10517-010-0902-7

Role of reactive oxygen species and Bcl-2 family proteins in TNF-α-induced apoptosis of lymphocytes

Bull Exp Biol Med. 2010 Aug;149(2):180-3. doi: 10.1007/s10517-010-0902-7.

[Article in English, Russian]

Authors

N V Ryazanceva¹, V V Novickiy, O B Zhukova, A K Biktasova, O E Chechina, E V Sazonova, M V Belkina, N Yu Chasovskih, Z K Khaitova

Affiliation

¹ Department for Fundamental Bases of Clinical Medicine, Siberian State Medical University, Russian Ministry of Health Care and Social Development, Tomsk, Russia. rnv@mail.ssmu.ru

PMID: 21113486
DOI: 10.1007/s10517-010-0902-7

Abstract

We studied the in vitro apoptosis-inducing effect of recombinant TNF-α (rTNF-α) on blood lymphocytes from healthy donors. rTNF-α-induced apoptosis was accompanied by an increase in the number of cells with low mitochondrial transmembrane potential, increased intracellular content of reactive oxygen species, reduced content of Bcl-2, Bcl-xL, and Bax proteins, and elevated Bad content. The molecular mechanisms of these changes are discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Apoptosis / physiology*
Caspases / metabolism
Female
Humans
In Vitro Techniques
Lymphocytes / cytology
Lymphocytes / physiology*
Male
Proto-Oncogene Proteins c-bcl-2 / metabolism
Reactive Oxygen Species / metabolism*
Receptors, Tumor Necrosis Factor / metabolism
Statistics, Nonparametric
Tumor Necrosis Factor-alpha / metabolism*

Substances

Proto-Oncogene Proteins c-bcl-2
Reactive Oxygen Species
Receptors, Tumor Necrosis Factor
Tumor Necrosis Factor-alpha
Caspases